Combination Therapy Using Fresh Cells and Cultured (Spheroid) Cells

1.Fresh Cells
(Freshly harvested stem cells)

2.Cultured Spheroid Cells
(Culture-expanded spheroid stem cells)

Medical Treatment: Two Types of Stem Cell Therapy

1.Fresh Cell Therapy
Fresh stem cells are extracted from approximately 100 cc of adipose tissue harvested under local anesthesia and administered to the affected area on the same day (outpatient procedure).
2.Cultured Spheroid Cell Therapy
A portion of the fresh cells is cryopreserved and cultured in our in-house Cell Processing Center (CPC) to form spheroids.
Approximately one month after the start of culture, the spheroid cells are administered to the affected area.
After the initial fresh cell injection, additional treatments are provided periodically based on the patient’s condition.

Treatment Features
• Fresh cells consist of a heterogeneous population of cells with diverse functions, including anti-inflammatory effects, angiogenesis, and tissue repair. Each cell type plays a specialized role.

• Subsequent treatment using spheroid cell aggregates is considered to provide greater therapeutic effects compared to administering individual dispersed cells.

• This combination therapy approach first suppresses inflammation, promotes blood vessel formation, and prepares the tissue environment for repair.
Following this, spheroid cell therapy further optimizes the environment and enhances tissue regeneration.

Indications / Conditions

1.Osteoarthritis
2.Rheumatoid Arthritis (refractory cases)
3.Injuries of muscles, tendons, ligaments, and other periarticular tissues

Providing Medical Institution

SOBAJIMA CLINIC

〒577-0011
Clinic court Higashino, 2-2-6 Aramoto-kita, Higashi Osaka City

https://sobacli-saisei.com/

Specialty

【Departments / Services】
Orthopedics (General)
Sports Orthopedics
Regenerative Medicine (Osteoarthritis)
Stroke & Lifestyle-related Diseases
Dementia
Rehabilitation
Breast Clinic
Various MRI Examinations

【Health Checkups】
Whole-body MRI Cancer Screening (DWIBS)
Painless Breast MRI Screening (DWIBS)
Brain Checkup (Head MRI & MRA)
Brain Checkup (Head MRI & MRA + Neck MRA)
Head & Cervical Spine Checkup
Breast Cancer Screening (Mammography + Breast Ultrasound + Physical Examination + Consultation)
Spine Checkup (Cervical & Lumbar MRI)
Osteoporosis Checkup (Thoracic & Lumbar MRI + Bone Density Test)
Brain Checkup with AI-based Dementia Risk Assessment

【Treatments】
PRP Therapy for Orthopedic Conditions
Extracorporeal Shockwave Therapy
Stem Cell Therapy (Orthopedic Conditions)
Stem Cell Therapy for Chronic Pain (e.g., numbness from spinal canal stenosis)
Stem Cell Therapy for Post-stroke Sequelae

Eligibility Criteria/Exclusions for Treatment

Contraindications (Not Eligible for Treatment) Patients who meet any of the following conditions are not eligible for treatment: • Patients receiving dual antiplatelet therapy • Patients for whom temporary discontinuation of antiplatelet agents is not possible • Patients who are deemed unsuitable for treatment based on preoperative evaluation by a physician

Remarks

【Program Introduction Video】 https://www.youtube.com/@そばじまクリニック 【Treatment Schedule】 Initial consultation and various examinations are conducted to determine treatment eligibility (approximately 1–2 weeks). If eligibility is confirmed, adipose tissue harvesting and fresh cell therapy are performed. The procedure is conducted on an outpatient basis (same-day surgery), followed by regular follow-up evaluations. At the time of fresh cell administration, a portion of the cells is cryopreserved in advance. For spheroid cell therapy, the cryopreserved cells are thawed as needed and cultured in our in-house Cell Processing Center (CPC). After several days of processing, cultured spheroid cell aggregates are prepared and administered (spheroid therapy). Spheroid therapy can be performed approximately one month or later after adipose tissue harvesting (fresh cell treatment).

Precautions / Contraindications

Understanding of the Safety and Efficacy of This Regenerative Medical Treatment